Brittany Grassbaugh

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Faculty Mentor: Michael Strano

Direct Supervisor: Nigel F. Reuel

Home University: California State University, Fullerton

Major: Biochemistry

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Biography

I have recently completed my first year at California State University, Fullerton as a transfer student majoring in Biochemistry. I am interested in almost all areas of scientific research and am hoping to use experiences, such as MSRP, to figure out exactly what type of research I would like to do in the future. I have currently been involved in a Molecular Biology lab researching plants and their defenses toward pathogens. Having lived my whole life in Southern California, I enjoy just about all outdoor activities including running, soccer, boating, and scuba diving.

Abstract

Design and fabrication of polyacrylamide gel platform with embedded SWNT sensors for detection of antibodies and glycans from cell extract supernatant

The fluorescent properties of single-walled carbon nanotubes (SWNT) are being utilized to design a biosensor for antibody screening for direct application in the field of biomanufacturing. To overcome time and sensitivity limitations of current screening methods, SWNT have been embedded within a layer of polyacrylamide gel and engineered to act as an optical switch upon binding of antibody or specific glycan classes. This platform will later be used in a multiplexed system for better resolved, high-throughput glycoprofiling. The fabrication of functional, porous polyacrylamide gels has been improved to detect the interaction of human and murine IgG containing supernatant from hybridoma, CHO, and proprietary fungal cell lines. The platform was also rendered specific to popular antibody glycoforms, such as high mannose species using naturally occurring plant lectins. Ultimately this platform could be used for glycoprofiling and determination of product concentration in less time and with less reagent than currently established methods such as liquid chromatography, mass spectrometry, and ELISA.