Eshan Dahal

.

Faculty Mentor: Robert Langer

Direct Supervisor: Nicolas Bertrand

Home University: New Mexico State University

Major: Physics

 .

Biography

I am currently studying physics at New Mexico State University as a senior. I was born and raised in Nepal. I initially thought to be a theoretical physicist when I came to the US in 2009. However, I slowly got inclined towards applied biosciences because I like the idea of connecting fundamental sciences and engineering in a new way to do the translational research. I am presently fascinated by the notion of taking nanoscale approaches to solve the biomedical problems. My general interest is in freestyle dancing, hiking, playing ping pong, motor-biking, and free falling.

Abstract

How do the Physicochemical Properties of Nanoparticles Influence their Biological Behavior?

Interactions of nanoparticles (NPs) with biological systems are complex. There are many factors that affect the rapid clearance of NPs by the mononuclear phagocyte system. One way to understand this is to study the influence of each NPs properties (such as size, charge, and surface properties) on their protein adsorption pattern. Here, we synthesized six distinct polymeric NPs to see how their physicochemical properties and the amount of protein adsorbed change upon exposure to plasma. These PLGA-PEG NPs of 50 nm and 100 nm size have different PEG densities. We characterized their size and zeta potential before and after incubating in plasma. BCA assay gave the quantitative measurement of extracted proteins from NPs surface. The relationships among the physicochemical properties and the amount of protein adsorbed were studied. This study will provide indispensable bases to study the protein corona using mass spectrometry and the biodistribution of NPs in vivo.